منابع مشابه
Dye loading with patch pipettes.
This protocol describes the loading of individual cells with fluorescent probes via patch pipettes. The patch-clamp methodology has been successfully used for single-cell dye labeling in cultured neurons, brain slices, and in vivo preparations. A broad range of dyes can be used with this loading technique. Markers for morphological reconstruction (e.g., Lucifer yellow); ion-sensitive indicator ...
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When separating out those metals which are normally deposited on the cathode, a direct analysis of the amount deposited can be gotten by weighing the cathode before and after completion of electro-ultrafiltration, provided the current applied is above the deposition potential of the metal being removed. The collodion solutions can be prepared so as to give varying porosity according to the meth...
متن کاملErgonomic evaluation of ten single-channel pipettes.
UNLABELLED Repetitive pipetting is a task that is associated with work-related musculoskeletal disorders of the hand and arm. OBJECTIVE The purpose of this study was to evaluate the usability and ergonomic performance of commercially available pipettes as determined by user ratings and objective measurements. PARTICIPANTS Participants were laboratory technicians and scientists at the Lawren...
متن کاملCleaning patch-clamp pipettes for immediate reuse
Patch-clamp recording has enabled single-cell electrical, morphological and genetic studies at unparalleled resolution. Yet it remains a laborious and low-throughput technique, making it largely impractical for large-scale measurements such as cell type and connectivity characterization of neurons in the brain. Specifically, the technique is critically limited by the ubiquitous practice of manu...
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Successful single-cell isolation is a primary step for subsequent chemical and biological analyses of single cells. Conventional single-cell isolation methods often encounter operational complexity, limited efficiency, deterioration of cell viability, incompetence in the isolation of a single-cell into nanoliter liquid, and/or inability to select single adherent cells with specific phenotypes. ...
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ژورنال
عنوان ژورنال: Journal of Industrial & Engineering Chemistry
سال: 1919
ISSN: 0095-9014,1943-2968
DOI: 10.1021/ie50109a018